Composite

Part:BBa_K4759211:Design

Designed by: Jianghua Chen   Group: iGEM23_Jiangnan-China   (2023-10-07)


T7-RBS3-hemH


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
    Illegal NgoMIV site found at 958
    Illegal AgeI site found at 103
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We cloned gene hemH to construct the plasmid to express it.

Source

E. coli genome

References